Pediatric Research

David A. Rosen, M.D., Ph.D. rosend@wustl.edu

Assistant Professor of Pediatrics

Researcher, Pathobiology

View my lab

Infectious Diseases Molecular Microbiology

phone: (314) 454-6050

Research Interests

Dr. Rosen evaluates children on the Infectious Diseases service at St. Louis Children's Hospital. In the research lab, he studies basic pathogenic mechanisms of Klebsiella pneumoniae. This organism often carries sets of genes that render it resistant to most, if not all, antibiotics available. Klebsiella, and other carbapenem-resistant Enterobacteriaceae (CRE), have recently been assigned a threat level of urgent by the CDC. Utilizing mouse models of pneumonia and urinary tract infection, Dr. Rosen dissects mechanisms by which Klebsiella pneumoniae infects the host. Specific interests includes differential regulation of virulence determinants, including type 1 pili and capsule, and adaptive immune responses to Klebsiella in the lung. The ultimate goal is to develop further understanding of Klebsiella pneumoniae pathogenesis, which may reveal alternative therapies to combat these resistant organisms.

Education

BS, Highest Distinction and Highest Honors, University of Michigan, 2002
MD, Washington University School of Medicine, 2010
PhD, Washington University School of Medicine, 2010

Training

Pediatric Internship and Residency, St. Louis Children's Hospital, 2010 - 2012
Pediatric Infectious Disease Fellowship, Washington University School of Medicine, St. Louis Children's Hospital, 2012 - 2016

Licensure and Board Certification

2012 - PresMO, Physician and Surgeon License
2013 - PresGeneral Pediatrics, American Board of Pediatrics
2017 - PresPediatric Infectious Diseases, American Board of Pediatrics

Honors and Awards

Farmington Rotary Foundation Daniel Waldman Memorial Scholarship, 1998
State of Michigan House of Representatives Certificate of Recognition, 1998
University of Michigan Regents-Alumni Scholarship, 1998
William Beaumont Hospital Scholarship, 1998 - 2001
University of Michigan James B. Angell Scholar, 1999 - 2002
National Stark Scholarship for Chemistry and Biochemistry, 2001
American Institute of Chemistry Merit Award for Biochemistry, 2002
Washington University Medical School Teaching Assistant of the Year, 2007
Washington University Teaching Citation, 2008
Alexander Berg Prize for Microbiology Research, 2010
St. Louis Children’s Hospital Chief Resident Award, 2012
Pediatric Infectious Diseases Society Fellowship Award, 2013 - 2015
Oliver Langenberg Physician-Scientist Training Program Post-Doctoral Trainee, Washington University School of Medicine, 2014 - 2017
St. Louis Children’s Hospital Outstanding Fellow Teaching Award, 2015 - 2016
Pediatric Infectious Diseases Society Fellow Video Award, 2015
Publons Peer Review Award, Top 1% in Microbiology, 2018
St. Jude/PIDS Pediatric Infectious Diseases Research Conference Poster Award, 2019
American Association of Immunology Early Career Travel Award, 2020

Selected Publications view all (12)

Publication Co-Authors

Gregory A. Storch, M.D.

David A. Hunstad, M.D.

Kristine Wylie, Ph.D.

Todd N. Wylie

S. Celeste Morley, M.D., Ph.D.

1.	Rosen DA, Twentyman J, Hunstad DA. High levels of cyclic di-GMP in Klebsiella pneumoniae attenuate virulence in the lung. Infect Immun. 2018;86(2). PMCID:PMC5778367 PMID:29158434
2.	Rosen DA, Hilliard JK, Tiemann KM, Todd EM, Morley SC, Hunstad DA. Klebsiella pneumoniae FimK promotes virulence in murine pneumonia. J Infect Dis. 2016;213(4):649-58. PMCID:PMC4721909 PMID:26347570
3.	Rosen DA, Pinkner JS, Jones JM, Walker JN, Clegg S, Hultgren SJ. Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression. Infect Immun. 2008;76(7):3337-45. PMCID:PMC2446714 PMID:18411285
4.	Rosen DA, Hooton TM, Stamm WE, Humphrey PA, Hultgren SJ. Detection of intracellular bacterial communities in human urinary tract infection. PLoS Med. 2007;4(12):e329. PMCID:PMC2140087 PMID:18092884

1.	Twentyman J, Morffy Smith C, Nims JS, Dahler AA, Rosen DA. A murine model demonstrates capsule-independent adaptive immune protection in survivors of Klebsiella pneumoniae respiratory tract infection. Dis Model Mech. 2020;13(3). PMCID:PMC7104859 PMID:32298236
2.	Feldman MF, Mayer Bridwell AE, Scott NE, Vinogradov E, McKee SR, Chavez SM, Twentyman J, Stallings CL, Rosen DA, Harding CM. A promising bioconjugate vaccine against hypervirulent Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 2019;116(37):18655-18663. PMCID:PMC6744904 PMID:31455739
3.	Schwartz DJ, Elward A, Storch GA, Rosen DA. Ureaplasma urealyticum pyelonephritis presenting with progressive dysuria, renal failure, and neurologic symptoms in an immunocompromised patient. Transpl Infect Dis. 2019;21(2):e13032. PMCID:PMC6542459 PMID:30472777
4.	Wylie KM, Wylie TN, Minx PJ, Rosen DA. Whole-genome sequencing of Klebsiella pneumoniae isolates to track strain progression in a single patient with recurrent urinary tract infection. Front Cell Infect Microbiol. 2019;9:14. PMCID:PMC6375827 PMID:30800643
5.	Potter RF, Lainhart W, Twentyman J, Wallace MA, Wang B, Burnham CA, Rosen DA, Dantas G. Population Structure, Antibiotic Resistance, and Uropathogenicity of Klebsiella variicola. MBio. 2018;9(6). PMCID:PMC6299229 PMID:30563902
6.	Svetlov D, Shi D, Twentyman J, Nedialkov Y, Rosen DA, Abagyan R, Artsimovitch I. In silico discovery of small molecules that inhibit RfaH recruitment to RNA polymerase. Mol Microbiol. 2018;110(1):128-142. PMCID:PMC6595482 PMID:30069925
7.	Rosen DA, Twentyman J, Hunstad DA. High levels of cyclic di-GMP in Klebsiella pneumoniae attenuate virulence in the lung. Infect Immun. 2018;86(2). PMCID:PMC5778367 PMID:29158434
8.	Rosen DA, Hilliard JK, Tiemann KM, Todd EM, Morley SC, Hunstad DA. Klebsiella pneumoniae FimK promotes virulence in murine pneumonia. J Infect Dis. 2016;213(4):649-58. PMCID:PMC4721909 PMID:26347570
9.	Rosen DA, Hung CS, Kline KA, Hultgren SJ. Streptozocin-induced diabetic mouse model of urinary tract infection. Infect Immun. 2008;76(9):4290-8. PMCID:PMC2519435 PMID:18644886
10.	Rosen DA, Pinkner JS, Walker JN, Elam JS, Jones JM, Hultgren SJ. Molecular variations in Klebsiella pneumoniae and Escherichia coli FimH affect function and pathogenesis in the urinary tract. Infect Immun. 2008;76(7):3346-56. PMCID:PMC2446687 PMID:18474655
11.	Rosen DA, Pinkner JS, Jones JM, Walker JN, Clegg S, Hultgren SJ. Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression. Infect Immun. 2008;76(7):3337-45. PMCID:PMC2446714 PMID:18411285
12.	Rosen DA, Hooton TM, Stamm WE, Humphrey PA, Hultgren SJ. Detection of intracellular bacterial communities in human urinary tract infection. PLoS Med. 2007;4(12):e329. PMCID:PMC2140087 PMID:18092884