Kristine Wylie, Ph.D.  kwylie@wustl.edu

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Assistant Professor of Pediatrics, Infectious Diseases
Infectious Diseases

phone: (314) 454-6050

Research Interests

Dr. Kristine Wylie is an Assistant Professor in the Department of Pediatrics at Washington University School of Medicine and the McDonnell Genome Institute. Her main research interest is the study of the dynamics of the microbiome and microbial infections during pregnancy. During Dr. Wylie’s postdoctoral work, she developed methods for analyzing high-throughput metagenomic shotgun sequence data sets to study the human virome, the viral component of the microbiome. Dr. Wylie analyzed the human virome (eukaryotic viruses) in the healthy subjects from the Human Microbiome Project, providing the first analysis of the commensal virome in a large cohort of generally healthy, asymptomatic people. She and her colleagues developed a targeted sequence capture panel, ViroCap, designed to enrich nucleic acid from eukaryotic DNA and RNA viruses from 34 families that infect vertebrate hosts. This approach greatly enhances the study of eukaryotic viruses using metagenomic sequencing. Using these methods, she and her colleagues have found that characteristics of the eukaryotic virome in the vagina associate with preterm birth. They are currently studying the dynamic associations between the vaginal microbiome (viruses and bacteria) and host response in a larger cohort of pregnant women and carrying out in vitro experiments to understand the mechanisms underlying associations with preterm birth.

Education

  • BA, Southern Illinois University1996
  • PhD, Saint Louis University2009

Training

  • Postdoctoral Research Associate, Washington University School of Medicine2009 - 2013

Honors and Awards

  • Michael R. Levy Award in Cellular and Molecular Biology, Southern Illinois University at Edwardsville1996
  • Sigma Xi, Saint Louis University2004
  • Presidential Fellowship, Saint Louis University2004 - 2008
  • Student travel award, American Society for Virology 27th Annual Meeting2008
  • Alpha Sigma Nu Jesuit Honor Society2009
  • Student travel award, American Society for Virology 28th Annual Meeting2009
  • Graduate Student of the Year, Molecular Microbiology and Immunology, Saint Louis University2009 - 2010
  • NHGRI Genome Advance of the Month, The Human Microbiome Project: Extending the definition of what constitutes a human2012

Selected Publications view all (58)


1.
Evaluation of patient- versus provider-collected vaginal swabs for microbiome analysis during pregnancy. BMC Res Notes. 2018;11(1):706. PMCID:PMC6173906  PMID:30290831 
2.
The vaginal eukaryotic DNA virome and preterm birth. Am J Obstet Gynecol. 2018. PMID:29738749 
3.
BreakPoint Surveyor: A Pipeline for Structural Variant Visualization. Bioinformatics. 2017. PMID:28582538 
4.
Early pregnancy vaginal microbiome trends and preterm birth. Am J Obstet Gynecol. 2017. PMID:28549981 
5.
Divergent viral presentation among human tumors and adjacent normal tissues Sci Rep. 2016;6:28294. doi:10.1038/srep28294  PMCID:MC4919655  PMID:27339696 
6.
Enhanced virome sequencing using targeted sequence capture. Genome Res. 2015;25(12):1910-20. doi:10.1101/gr.191049.115  PMID:26395152 
7.
Metagenomic analysis of double-stranded DNA viruses in healthy adults. BMC Biol. 2014;12(1):71. doi:10.1186/s12915-014-0071-7  PMCID:PMC4177058  PMID:25212266 
8.
Virome genomics: a tool for defining the human virome. Curr Opin Microbiol. 2013;16(4):479-84. doi:10.1016/j.mib.2013.04.006  PMCID:PMC3755052  PMID:23706900 
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