Pediatric Research

Todd Druley, M.D., Ph.D. druley_t@wustl.edu

Associate Professor of Pediatrics, Hematology and Oncology

View my lab

Hematology and Oncology

phone: (314) 454-6018

Research Interests

Dr. Druley's lab used computational and molecular biology to characterize the role of germline genetic variation in patient-specific inducible pluripotent stem cell models in predisposing to pediatric cancer.

Education

PhD, University of Illinois, 2002
MD, University of Illinois, 2002
BS, Departmental Distinction for Research, University of Illinois, 1994

Training

Resident, Washington University, 2002 - 2005
Clinical Fellow in Pediatric Hematology and Oncology, Washington University, 2005 - 2008

Licensure and Board Certification

2006 - 2016American Board of Pediatrics - General Pediatrics
2008 - PresMO
2013 - PresAmerican Board of Pediatrics - Pediatric Hematology and Oncology

Honors

Children Of Vietnam Veterans Merit Scholarship, 1990 - 1994
Research Distinction in Cell and Structural Biology, University of Illinois, 1994
Outstanding Fellow Teaching Award, 2006
Alex's Lemonade Stand "A" Award, 2009 - 2013
Scholar of the NIH Child Health Resource Center in Developmental Biology, 2009 - Pres
Children's Discovery Institute Faculty Scholar, 2010 - Pres
Mark Johnston Scholar in Genomic Science, 2010 - Pres
Hyundai Hope Award Recipient, 2011 - 2013
Doris Duke Clinical Scientist Development Award, 2014 - 2017
Best Poster at 2016 International Society of Pediatric Oncology (SIOP) Annual Meeting (Dublin, Ireland), 2016
Elected to the American Society of Clinical Investigators (ASCI), 2018 - Pres

Selected Publications view all (49)

Publication Co-Authors

David H. Gutmann, MD, PhD, FAAN

Shalini Shenoy, M.D.

Matthew I. Goldsmith, M.D.

Marwan Shinawi, M.D.

Keith A. Hruska, M.D.

Laura Schuettpelz, M.D, Ph.D.

S. Paul Hmiel, M.D., Ph.D.

Jennifer A Wambach, M.D.

Thomas Ferkol, M.D.

Mario Castro

Amjad Horani, M.D.

Joshua B. Rubin, M.D., Ph.D.

Beth A. Kozel, M.D., Ph.D.

Alok Kothari, M.D.

Leonard B. Bacharier, M.D.

Schwartz, Alan L., Ph.D., M.D.

Robert J. Hayashi, M.D.

Francis Sessions Cole, III, M.D.

1.	Druley TE, Wang L, Lin SJ, Lee JH, Zhang Q, Daw EW, Abel HJ, Chasnoff SE, Ramos EI, Levinson BT, Thyagarajan B, Newman AB, Christensen K, Mayeux R, Province MA. Candidate gene resequencing to identify rare, pedigree-specific variants influencing healthy aging phenotypes in the long life family study. BMC Geriatr. 2016;16(1):80. doi:10.1186/s12877-016-0253-y PMID:27060904
2.	Torgerson DG, Giri T, Druley TE, Zheng J, Huntsman S, Seibold MA, Young AL, Schweiger T, Yin-Declue H, Sajol GD, Schechtman KB, Hernandez RD, Randolph AG, Bacharier LB, Castro M. Pooled Sequencing of Candidate Genes Implicates Rare Variants in the Development of Asthma Following Severe RSV Bronchiolitis in Infancy. PLoS One. 2015;10(11):e0142649. PMCID:PMC4654486 PMID:26587832
3.	Madden LM, Ngwube AI, Shenoy S, Druley TE, Hayashi RJ. Late toxicity of a novel allogeneic stem cell transplant using single fraction total body irradiation for hematologic malignancies in children. J Pediatr Hematol Oncol. 2015;37(2):e94-e101. doi:10.1097/MPH.0000000000000272 PMCID:PMC4337424 PMID:25333837
4.	Wong TN, Ramsingh G, Young AL, Miller CA, Touma W, Welch JS, Lamprecht TL, Shen D, Hundal J, Fulton RS, Heath S, Baty JD, Klco JM, Ding L, Mardis ER, Westervelt P, DiPersio JF, Walter MJ, Graubert TA, Ley TJ, Druley TE, Link DC, Wilson RK. Role of TP53 mutations in the origin and evolution of therapy-related acute myeloid leukaemia. Nature. 2015;518(7540):552-5. doi:10.1038/nature13968 PMCID:PMC4403236 PMID:25487151
5.	Young AL, Wong TN, Hughes AE, Heath SE, Ley TJ, Link DC, Druley TE. Quantifying ultra-rare pre-leukemic clones via targeted error-corrected sequencing. Leukemia. 2015. doi:10.1038/leu.2015.17 PMID:25644247
6.	Warrington NM, Sun T, Luo J, McKinstry RC, Parkin PC, Ganzhorn S, Spoljaric D, Albers AC, Merkelson A, Stewart DR, Stevenson DA, Viskochil D, Druley TE, Forys JT, Reilly KM, Fisher MJ, Tabori U, Allen JC, Schiffman JD, Gutmann DH, Rubin JB. The cyclic AMP pathway is a sex-specific modifier of glioma risk in type I neurofibromatosis patients. Cancer Res. 2015;75(1):16-21. doi:10.1158/0008-5472.CAN-14-1891 PMCID:PMC4286430 PMID:25381154
7.	Valentine MC, Linabery AM, Chasnoff S, Hughes AE, Mallaney C, Sanchez N, Giacalone J, Heerema NA, Hilden JM, Spector LG, Ross JA, Druley TE. Excess congenital non-synonymous variation in leukemia-associated genes in MLL- infant leukemia: a Children's Oncology Group report. Leukemia. 2014;28(6):1235-41. doi:10.1038/leu.2013.367 PMCID:PMC4045651 PMID:24301523
8.	Wambach JA, Wegner DJ, Heins HB, Druley TE, Mitra RD, Hamvas A, Cole FS. Synonymous ABCA3 variants do not increase risk for neonatal respiratory distress syndrome. J Pediatr. 2014;164(6):1316-21.e3. doi:10.1016/j.jpeds.2014.02.021 PMCID:PMC4035386 PMID:24657120
9.	Ramos EI, Bien-Willner GA, Li J, Hughes AE, Giacalone J, Chasnoff S, Kulkarni S, Parmacek M, Cole FS, Druley TE. Genetic variation in MKL2 and decreased downstream PCTAIRE1 expression in extreme, fatal primary human microcephaly. Clin Genet. 2014;85(5):423-32. doi:10.1111/cge.12197 PMCID:PMC3929543 PMID:23692340

1.	Camet ML, Hayashi SS, Druley T, Henry J, Gettinger K, Stacy A, Hayashi RJ. Scope of hearing loss in Beckwith-Wiedemann syndrome and hemihypertrophy. Am J Med Genet A. 2019. PMID:31339626
2.	Golyan FF, Druley TE, Abbaszadegan MR. Whole-exome sequencing of familial esophageal squamous cell carcinoma identified rare pathogenic variants in new predisposition genes. Clin Transl Oncol. 2019. PMID:31321674
3.	Young AL, Tong RS, Birmann BM, Druley TE. Clonal haematopoiesis and risk of acute myeloid leukemia. Haematologica. 2019. PMID:31004019
4.	Sadler B, Haller G, Antunes L, Bledsoe X, Morcuende J, Giampietro P, Raggio C, Miller N, Kidane Y, Wise CA, Amarillo I, Walton N, Seeley M, Johnson D, Jenkins C, Jenkins T, Oetjens M, Tong RS, Druley TE, Dobbs MB, Gurnett CA. Distal chromosome 16p11.2 duplications containing SH2B1 in patients with scoliosis. J Med Genet. 2019. PMID:30803986
5.	Uli N, Michelen-Gomez E, Ramos EI, Druley TE. Age-specific changes in genome-wide methylation enrich for Foxa2 and estrogen receptor alpha binding sites. PLoS One. 2018;13(9):e0203147. PMCID:PMC6157835 PMID:30256791
6.	Wong WH, Tong RS, Young AL, Druley TE. Rare Event Detection Using Error-corrected DNA and RNA Sequencing. J Vis Exp. 2018;(138). PMID:30124656
7.	Groves AP, Gettinger K, Druley TE, A Kozel B, Shinawi M, Mohrmann C, Henry J, Jacobi C, Trinkaus K, Hayashi RJ. Special Therapy and Psychosocial Needs Identified in a Multidisciplinary Cancer Predisposition Syndrome Clinic. J Pediatr Hematol Oncol. 2018. PMID:30028825
8.	Azar C, Valentine M, Trausch-Azar J, Druley T, Nelson DM, Schwartz AL. RNA-Seq identifies genes whose proteins are transformative in the differentiation of cytotrophoblast to syncytiotrophoblast, in human primary villous and BeWo trophoblasts. Sci Rep. 2018;8(1):5142. PMCID:PMC5865118 PMID:29572450
9.	Marcotte EL, Druley TE, Johnson KJ, Richardson M, von Behren J, Mueller BA, Carozza S, McLaughlin C, Chow EJ, Reynolds P, Spector LG. Parental Age and Risk of Infant Leukaemia: A Pooled Analysis. Paediatr Perinat Epidemiol. 2017. PMID:28940632
10.	Wong-Siegel JR, Johnson KJ, Gettinger K, Cousins N, McAmis N, Zamarione A, Druley TE. Congenital neurodevelopmental anomalies in pediatric and young adult cancer. Am J Med Genet A. 2017. PMID:28851129
11.	Johnson KJ, Lee JM, Ahsan K, Padda H, Feng Q, Partap S, Fowler SA, Druley TE. Pediatric cancer risk in association with birth defects: A systematic review. PLoS One. 2017;12(7):e0181246. PMID:28749971
12.	Kalish JM, Doros L, Helman LJ, Hennekam RC, Kuiper RP, Maas SM, Maher ER, Nichols KE, Plon SE, Porter CC, Rednam S, Schultz KAP, States LJ, Tomlinson GE, Zelley K, Druley TE. Surveillance Recommendations for Children with Overgrowth Syndromes and Predisposition to Wilms Tumors and Hepatoblastoma. Clin Cancer Res. 2017;23(13):e115-e122. PMCID:PMC5538793 PMID:28674120
13.	Porter CC, Druley TE, Erez A, Kuiper RP, Onel K, Schiffman JD, Wolfe Schneider K, Scollon SR, Scott HS, Strong LC, Walsh MF, Nichols KE. Recommendations for Surveillance for Children with Leukemia-Predisposing Conditions. Clin Cancer Res. 2017;23(11):e14-e22. PMID:28572263
14.	Kalish JM, Biesecker LG, Brioude F, Deardorff MA, Di Cesare-Merlone A, Druley T, Ferrero GB, Lapunzina P, Larizza L, Maas S, Macchiaiolo M, Maher ER, Maitz S, Martinez-Agosto JA, Mussa A, Robinson P, Russo S, Selicorni A, Hennekam RC. Nomenclature and definition in asymmetric regional body overgrowth. Am J Med Genet A. 2017. PMID:28475229
15.	Creamer JP, Dege C, Ren Q, Ho JT, Valentine MC, Druley TE, Sturgeon CM. Human definitive hematopoietic specification from pluripotent stem cells is regulated by mesodermal expression of CDX4. Blood. 2017. PMID:28408465
16.	Kramer AC, Kothari A, Wilson WC, Celik H, Nikitas J, Mallaney C, Ostrander EL, Eultgen E, Martens A, Valentine MC, Young AL, Druley TE, Figueroa ME, Zhang B, Challen GA. Dnmt3a regulates T-cell development and suppresses T-ALL transformation. Leukemia. 2017. PMID:28321121
17.	Young AL, Challen GA, Birmann BM, Druley TE. Clonal haematopoiesis harbouring AML-associated mutations is ubiquitous in healthy adults. Nat Commun. 2016;7:12484. PMCID:PMC4996934 PMID:27546487
18.	Druley, TE. The relative contributions of germline variation, epimutation, and somatic mutation to paediatric leukemia predisposition. EMJ Hematol. 2016;4(1):110-116.
19.	Druley TE, Wang L, Lin SJ, Lee JH, Zhang Q, Daw EW, Abel HJ, Chasnoff SE, Ramos EI, Levinson BT, Thyagarajan B, Newman AB, Christensen K, Mayeux R, Province MA. Candidate gene resequencing to identify rare, pedigree-specific variants influencing healthy aging phenotypes in the long life family study. BMC Geriatr. 2016;16(1):80. doi:10.1186/s12877-016-0253-y PMID:27060904
20.	Torgerson DG, Giri T, Druley TE, Zheng J, Huntsman S, Seibold MA, Young AL, Schweiger T, Yin-Declue H, Sajol GD, Schechtman KB, Hernandez RD, Randolph AG, Bacharier LB, Castro M. Pooled Sequencing of Candidate Genes Implicates Rare Variants in the Development of Asthma Following Severe RSV Bronchiolitis in Infancy. PLoS One. 2015;10(11):e0142649. PMCID:PMC4654486 PMID:26587832
21.	Hing B, Ramos E, Braun P, McKane M, Jancic D, Tamashiro KL, Lee RS, Michaelson JJ, Druley TE, Potash JB. Adaptation of the targeted capture Methyl-Seq platform for the mouse genome identifies novel tissue-specific DNA methylation patterns of genes involved in neurodevelopment. Epigenetics. 2015;10(7):581-96. doi:10.1080/15592294.2015.1045179. PMCID:PMC4622595 PMID:25985232
22.	Madden LM, Ngwube AI, Shenoy S, Druley TE, Hayashi RJ. Late toxicity of a novel allogeneic stem cell transplant using single fraction total body irradiation for hematologic malignancies in children. J Pediatr Hematol Oncol. 2015;37(2):e94-e101. doi:10.1097/MPH.0000000000000272 PMCID:PMC4337424 PMID:25333837
23.	Wong TN, Ramsingh G, Young AL, Miller CA, Touma W, Welch JS, Lamprecht TL, Shen D, Hundal J, Fulton RS, Heath S, Baty JD, Klco JM, Ding L, Mardis ER, Westervelt P, DiPersio JF, Walter MJ, Graubert TA, Ley TJ, Druley TE, Link DC, Wilson RK. Role of TP53 mutations in the origin and evolution of therapy-related acute myeloid leukaemia. Nature. 2015;518(7540):552-5. doi:10.1038/nature13968 PMCID:PMC4403236 PMID:25487151
24.	Young AL, Wong TN, Hughes AE, Heath SE, Ley TJ, Link DC, Druley TE. Quantifying ultra-rare pre-leukemic clones via targeted error-corrected sequencing. Leukemia. 2015. doi:10.1038/leu.2015.17 PMID:25644247
25.	Warrington NM, Sun T, Luo J, McKinstry RC, Parkin PC, Ganzhorn S, Spoljaric D, Albers AC, Merkelson A, Stewart DR, Stevenson DA, Viskochil D, Druley TE, Forys JT, Reilly KM, Fisher MJ, Tabori U, Allen JC, Schiffman JD, Gutmann DH, Rubin JB. The cyclic AMP pathway is a sex-specific modifier of glioma risk in type I neurofibromatosis patients. Cancer Res. 2015;75(1):16-21. doi:10.1158/0008-5472.CAN-14-1891 PMCID:PMC4286430 PMID:25381154
26.	Valentine MC, Linabery AM, Chasnoff S, Hughes AE, Mallaney C, Sanchez N, Giacalone J, Heerema NA, Hilden JM, Spector LG, Ross JA, Druley TE. Excess congenital non-synonymous variation in leukemia-associated genes in MLL- infant leukemia: a Children's Oncology Group report. Leukemia. 2014;28(6):1235-41. doi:10.1038/leu.2013.367 PMCID:PMC4045651 PMID:24301523
27.	Wambach JA, Wegner DJ, Heins HB, Druley TE, Mitra RD, Hamvas A, Cole FS. Synonymous ABCA3 variants do not increase risk for neonatal respiratory distress syndrome. J Pediatr. 2014;164(6):1316-21.e3. doi:10.1016/j.jpeds.2014.02.021 PMCID:PMC4035386 PMID:24657120
28.	Ramos EI, Bien-Willner GA, Li J, Hughes AE, Giacalone J, Chasnoff S, Kulkarni S, Parmacek M, Cole FS, Druley TE. Genetic variation in MKL2 and decreased downstream PCTAIRE1 expression in extreme, fatal primary human microcephaly. Clin Genet. 2014;85(5):423-32. doi:10.1111/cge.12197 PMCID:PMC3929543 PMID:23692340
29.	Kanchi KL, Johnson KJ, Lu C, McLellan MD, Leiserson MD, Wendl MC, Zhang Q, Koboldt DC, Xie M, Kandoth C, McMichael JF, Wyczalkowski MA, Larson DE, Schmidt HK, Miller CA, Fulton RS, Spellman PT, Mardis ER, Druley TE, Graubert TA, Goodfellow PJ, Raphael BJ, Wilson RK, Ding L. Integrated analysis of germline and somatic variants in ovarian cancer. Nat Commun. 2014;5:3156. doi:10.1038/ncomms4156 PMCID:PMC4025965 PMID:24448499
30.	Alvarado DM, Yang P, Druley TE, Lovett M, Gurnett CA. Multiplexed direct genomic selection (MDiGS): a pooled BAC capture approach for highly accurate CNV and SNP/INDEL detection. Nucleic Acids Res. 2014;42(10):e82. doi:10.1093/nar/gku218 PMCID:PMC4041413 PMID:24682816
31.	Druley TE. Commentary. Clin Chem. 2013;59(1):50-1. PMID:23284014
32.	Lee JH, Cheng R, Honig LS, Feitosa M, Kammerer CM, Kang MS, Schupf N, Lin SJ, Sanders JL, Bae H, Druley T, Perls T, Christensen K, Province M, Mayeux R. Genome wide association and linkage analyses identified three loci-4q25, 17q23.2, and 10q11.21-associated with variation in leukocyte telomere length: the Long Life Family Study. Front Genet. 2013;4:310. doi:10.3389/fgene.2013.00310 PMCID:PMC3894567 PMID:24478790
33.	Ramos E, Levinson BT, Chasnoff S, Hughes A, Young AL, Thornton K, Li A, Vallania FL, Province M, Druley TE. Population-based rare variant detection via pooled exome or custom hybridization capture with or without individual indexing. BMC Genomics. 2012;13:683. doi:10.1186/1471-2164-13-683 PMCID:PMC3534616 PMID:23216810
34.	Wambach JA, Wegner DJ, Depass K, Heins H, Druley TE, Mitra RD, An P, Zhang Q, Nogee LM, Cole FS, Hamvas A. Single ABCA3 mutations increase risk for neonatal respiratory distress syndrome. Pediatrics. 2012;130(6):e1575-82. doi:10.1542/peds.2012-0918 PMCID:PMC3507255 PMID:23166334
35.	Chatterjee R, Ramos E, Hoffman M, VanWinkle J, Martin DR, Davis TK, Hoshi M, Hmiel SP, Beck A, Hruska K, Coplen D, Liapis H, Mitra R, Druley T, Austin P, Jain S. Traditional and targeted exome sequencing reveals common, rare and novel functional deleterious variants in RET-signaling complex in a cohort of living US patients with urinary tract malformations. Hum Genet. 2012;131(11):1725-38. doi:10.1007/s00439-012-1181-3 PMCID:PMC3551468 PMID:22729463
36.	Horani A, Druley TE, Zariwala MA, Patel AC, Levinson BT, Van Arendonk LG, Thornton KC, Giacalone JC, Albee AJ, Wilson KS, Turner EH, Nickerson DA, Shendure J, Bayly PV, Leigh MW, Knowles MR, Brody SL, Dutcher SK, Ferkol TW. Whole-exome capture and sequencing identifies HEATR2 mutation as a cause of primary ciliary dyskinesia. Am J Hum Genet. 2012;91(4):685-93. doi:10.1016/j.ajhg.2012.08.022 PMCID:PMC3484505 PMID:23040496
37.	Vallania F, Ramos E, Cresci S, Mitra RD, Druley TE. Detection of rare genomic variants from pooled sequencing using SPLINTER. J Vis Exp. 2012;(64). doi:10.3791/3943 PMCID:PMC3471313 PMID:22760212
38.	Haller G, Druley T, Vallania FL, Mitra RD, Li P, Akk G, Steinbach JH, Breslau N, Johnson E, Hatsukami D, Stitzel J, Bierut LJ, Goate AM. Rare missense variants in CHRNB4 are associated with reduced risk of nicotine dependence. Hum Mol Genet. 2012;21(3):647-55. doi:10.1093/hmg/ddr498 PMCID:PMC3259016 PMID:22042774
39.	Vallania FL, Druley TE, Ramos E, Wang J, Borecki I, Province M, Mitra RD. High-throughput discovery of rare insertions and deletions in large cohorts. Genome Res. 2010;20(12):1711-8. doi:10.1101/gr.109157.110 PMCID:PMC2989997 PMID:21041413
40.	Ziga ED, Druley T, Burnham CA. Herbaspirillum species bacteremia in a pediatric oncology patient. J Clin Microbiol. 2010;48(11):4320-1. doi:10.1128/JCM.01479-10 PMCID:PMC3020882 PMID:20739483
41.	Gurnett CA, Desruisseau DM, McCall K, Choi R, Meyer ZI, Talerico M, Miller SE, Ju JS, Pestronk A, Connolly AM, Druley TE, Weihl CC, Dobbs MB. Myosin binding protein C1: a novel gene for autosomal dominant distal arthrogryposis type 1. Hum Mol Genet. 2010;19(7):1165-73. doi:10.1093/hmg/ddp587 PMCID:PMC2838534 PMID:20045868
42.	Matkovich SJ, Van Booven DJ, Hindes A, Kang MY, Druley TE, Vallania FL, Mitra RD, Reilly MP, Cappola TP, Dorn GW 2nd. Cardiac signaling genes exhibit unexpected sequence diversity in sporadic cardiomyopathy, revealing HSPB7 polymorphisms associated with disease. J Clin Invest. 2010;120(1):280-9. doi:10.1172/JCI39085 PMCID:PMC2798680 PMID:20038796
43.	Schuettpelz LG, Behrens D, Goldsmith MI, Druley TE. Severe ceftriaxone-induced hemolysis complicated by diffuse cerebral ischemia in a child with sickle cell disease. J Pediatr Hematol Oncol. 2009;31(11):870-2. doi:10.1097/MPH.0b013e3181b7eda2 PMID:19829151
44.	Druley TE, Vallania FL, Wegner DJ, Varley KE, Knowles OL, Bonds JA, Robison SW, Doniger SW, Hamvas A, Cole FS, Fay JC, Mitra RD. Quantification of rare allelic variants from pooled genomic DNA. Nat Methods. 2009;6(4):263-5. doi:10.1038/nmeth.1307 PMCID:PMC2776647 PMID:19252504
45.	Druley TE, Hayashi R, Mansur DB, Zhang QJ, Barnes Y, Trinkaus K, Witty S, Thomas T, Klein EE, DiPersio JF, Adkins D, Shenoy S. Early outcomes after allogeneic hematopoietic SCT in pediatric patients with hematologic malignancies following single fraction TBI. Bone Marrow Transplant. 2009;43(4):307-14. doi:10.1038/bmt.2008.327 PMCID:PMC2792985 PMID:19011666
46.	Litman T, Druley TE, Stein WD, Bates SE. From MDR to MXR: new understanding of multidrug resistance systems, their properties and clinical significance. Cell Mol Life Sci. 2001;58(7):931-59. doi:10.1007/PL00000912 PMID:11497241
47.	Druley TE, Stein WD, Roninson IB. Analysis of MDR1 P-glycoprotein conformational changes in permeabilized cells using differential immunoreactivity. Biochemistry. 2001;40(14):4312-22. PMID:11284687
48.	Druley TE, Stein WD, Ruth A, Roninson IB. P-glycoprotein-mediated colchicine resistance in different cell lines correlates with the effects of colchicine on P-glycoprotein conformation. Biochemistry. 2001;40(14):4323-31. PMID:11284688
49.	Mechetner EB, Schott B, Morse BS, Stein WD, Druley T, Davis KA, Tsuruo T, Roninson IB. P-glycoprotein function involves conformational transitions detectable by differential immunoreactivity. Proc Natl Acad Sci U S A. 1997;94(24):12908-13. PMCID:PMC24237 PMID:9371774